Copyright © 2020 Elsevier B.V. or its licensors or contributors. Protein-nucleic acid interactions and DNA conformation in a complex of human immunodeficiency virus type 1 reverse transcriptase with a double-stranded DNA template-primer. Moloney murine leukemia virus (MMLV) reverse transcriptase (RT).
Protein-DNA interactions, which are nearly identical in each of the three lattices, involve four conserved residues in the fingers domain, Asp114, Arg116, Asn119 and Gly191.
Gu J, Villanueva RA, Snyder CS, Roth MJ, Georgiadis MM. Acta Crystallogr D Biol Crystallogr. Get the latest research from NIH: https://www.nih.gov/coronavirus. Authors S Najmudin 1 , M L Coté, D Sun, S Yohannan, S P Montano, J Gu, M M Georgiadis. Reverse transcriptase from Moloney murine leukemia virus (MMLVRT) is an RNA dependent DNA polymerase, which has been used as a fundamental tool for molecular biology and biotechnology. | |
2001 Jan 12;305(2):341-59. doi: 10.1006/jmbi.2000.4281. Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 A resolution.
DNA atoms involved in the interactions include the 3'-OH group from the primer strand and minor groove base atoms and sugar atoms from the n-2 and n-3 positions of the template strand, where n is the template base that would pair with an incoming nucleotide. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error.
Acta Crystallogr D Biol Crystallogr.
Through site-directed mutagenesis, we have substituted residues that are involved in binding DNA in our crystal structures and have characterized the resulting enzymes. Taken together, these results demonstrate that silkworm-BEVS is a promising alternative strategy to produce the functional and thermostable reverse transcriptase. Reverse transcriptase (RT) serves as the replicative polymerase for retroviruses by using RNA and DNA-directed DNA polymerase activities coupled with a ribonuclease H activity to synthesize a double-stranded DNA copy of the single-stranded RNA genome. We now propose that nucleic acid binding to the fingers domain may play a role in translocation of nucleic acid during processive DNA synthesis and suggest that our complex may represent an intermediate in this process. USA.gov. 2014 Apr;21(4):389-96. doi: 10.1038/nsmb.2785. Moloney murine leukemia virus reverse transcriptase (MMLVRT) was expressed in silkworm-baculovirus expression vector system.
Published by Elsevier B.V. All rights reserved. Get the latest public health information from CDC: https://www.coronavirus.gov. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. Here, we will review the similarities and differences between MoMLV RT and HIV-1 RT via structural analysis, and propose working models for the MoMLV RT based upon that information.
Previous biochemical studies of MoMLV RT in conjunction with knowledge of sequence homologies to HIV-1 RT and overall fold similarities to RTs in general, provided a foundation upon which to build. Now in the advent of new structural information, more intricate examination of MoMLV RT in its entirety can be realized, and thus the comparisons with HIV-1 RT may be more critically elucidated.
2017 Jun 29;15(1):54. doi: 10.1186/s12915-017-0387-1. Biopolymers. Ding J, Das K, Hsiou Y, Sarafianos SG, Clark AD Jr, Jacobo-Molina A, Tantillo C, Hughes SH, Arnold E. J Mol Biol. 2014 Mar;70(Pt 3):752-9. doi: 10.1107/S1399004713032987. J Mol Biol. The secondary structures formed in the RNA templates decrease the accessibility of the reverse transcriptase to the RNA templates; it is important to unfold the RNA secondary structure by increasing the reaction temperature to perform the successful transcription. Introduction.
Für ihre Entdeckung erhielten beide 1975 den Nobelpreis für Physiologie oder Medizin, gemeinsam mit Renato Dulbecco.Die Erzeugung von cDNA aus RNA mit Hilfe von reversen … A 190-base-pair DNA-RNA hybrid containing the Moloney murine leukemia virus origin of plus-strand DNA synthesis was constructed and used as a source of template-primer for the reverse transcriptase in vitro. 1998 Dec 11;284(4):1095-111. doi: 10.1006/jmbi.1998.2208. Reverse Transkriptasen wurden 1970 gleichzeitig durch Howard M. Temin im Rous-Sarkom-Virus (RSV) und durch David Baltimore im RSV und im Moloney Murine Leukemia Virus (MoMLV) entdeckt.
Structural and energetic characterization of nucleic acid-binding to the fingers domain of Moloney murine leukemia virus reverse transcriptase.
Recombinant MMLVRT enzymes showed intact RNA-dependent DNA polymerase activity. Nowak E, Miller JT, Bona MK, Studnicka J, Szczepanowski RH, Jurkowski J, Le Grice SF, Nowotny M. Nat Struct Mol Biol. To determine the various roles of RNase H in reverse transcription, we generated a panel of mutations in the RNase H domain of Moloney murine leukemia virus reverse transcriptase based on sequence alignments and the crystal structures of Escherichia coli and human immunodeficiency virus type 1 RNases H (S. W. Blain and S. P. Goff, J. Biol.
Epub 2013 Feb 4. Proteins. Synthesis was shown to initiate precisely at the known plus-strand origin. In an effort to obtain detailed structural information about nucleic acid interactions with reverse transcriptase, we have determined crystal structures at 2.3 A resolution of an N-terminal fragment from Moloney murine leukemia virus reverse transcriptase complexed to blunt-ended DNA in three distinct lattices.
Moloney MLV (Mo-MLV, auch Mo-MuLV, M-MuLV), benannt nach John B. Moloney, (1960). https://doi.org/10.1016/j.virusres.2008.01.001. Skariah G, Seimetz J, Norsworthy M, Lannom MC, Kenny PJ, Elrakhawy M, Forsthoefel C, Drnevich J, Kalsotra A, Ceman S. BMC Biol.
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